Characterisation of hepatic lipid signature distributed across the liver zonation using mass spectrometry imaging
Highlights
We characterised the hepatic lipid distribution across liver zonation using DESI-MSI.
Fatty acids were predominantly detected in the periportal region, whereas triacylglycerols were detected mainly in the pericentral region.
Although phospholipids were distributed in the periportal and pericentral zones, phosphatidylinositols were located predominantly in the midzone.
De novo triacylglycerol biosynthesis appeared to be the most influenced pathway across the three zones.
Assessing zone-specific hepatic lipid metabolism may lead to a better understanding of lipid homeostasis during disease progression.
Comparison of the liver zonation pattern using liver histology, immunofluorescence imaging, and molecular imaging with DESI. (A) H&E staining of liver tissue after DESI-MSI analysis. (B) Double immunofluorescence staining shows the proto-central axis along with the liver zonation. GS-6, pericentral hepatocytes (blue); E-Cad, periportal hepatocytes (red); and DAPI (yellow). (C) Red, green, and blue overlay ion image from DESI-MSI: PI(38:3) located in the pericentral area (blue); PI(36:3) mainly expressed in the midzone (green); and PE(38:6) predominantly presented in the periportal region (red). CV, central vein; DESI, desorption electrospray ionisation; DESI-MSI, desorption electrospray ionisation mass spectrometry imaging; E-Cad, E-cadherin; GS-6, glutamine synthetase; MZ, midzone; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PV, portal vein.
Link to full text: https://www.jhep-reports.eu/article/S2589-5559(23)00056-3/fulltext
Seubnooch, P., Montani, M., Tsouka, S., Claude, E., Rafiqi, U., Perren, A., Dufour, J.-F., & Masoodi, M. (2023). Characterisation of hepatic lipid signature distributed across the liver zonation using mass spectrometry imaging. In JHEP Reports (Vol. 5, Issue 6, p. 100725). Elsevier BV. https://doi.org/10.1016/j.jhepr.2023.100725